Research Interests:
My research interests lie in the regulation of the stress response in bees. I have been studying transcription factors that aid in the survival of invasive bees in conditions where honey bees would normally not survive. Students working under my supervisions have cloned, sequenced and characterized expression of factors linked to stress response and survival, daf-16, hsf-1, and nrf2 in Megachile rotundata, a solitary thermotolerant bee. Summer of 2017, we also looked at expression of a hypoxia related factor, hif1α, in response to heat stress. This past year, in collaboration with Dr. John Hranitz, we conducted experiments to verify changes in genes linked to stress, behavior and metabolism in honey bees in response to ethanol.
In 2016, I applied for a yearlong sabbatical, which was approved for the Spring/Fall 2018 semesters. In fall of 2017, my students and I treated honey bees with ethanol (or control) and isolated RNA for real time PCR experiments. We conducted these experiments to study gene expression changes indicated by an initial microarray experiment. In the spring/fall 2018 semesters, my students and I conducted real- time PCR experiments to verify gene expression changes in hsc70-4, nrf2, egln1 and foxp2 to name a few genes, in the ethanol treated bee samples. One of my students, Justin Heller, decided to work with me on his Master’s degree pursuing genes in alcohol tolerance starting the fall 2018 semester.
For more information on Dr. Brubaker's work visit her faculty profile.